lowry based bio rad dc protein assay kit  (Bio-Rad)

Journal: Frontiers in Physiology

Article Title: Identification of transient receptor potential melastatin 3 proteotypic peptides employing an efficient membrane protein extraction method for natural killer cells

doi: 10.3389/fphys.2022.947723

Figure Lengend Snippet: Determination of protein extraction efficiency by the two adapted protocols. (A) Comparison of average total membrane protein yield from cultured K562 cells using the two adapted protocols. 2 × 10 6 K562 cells were used per sample. For both protocols, protein quantification was performed using the Pierce BCA and Bio-Rad DC protein assays simultaneously using a NanoDrop spectrophotometer. Normality was determined using the Shapiro Wilk test ( p > 0.05). Data is represented as the mean ± SEM as determined by independent t -test. * p < 0.05. (B) Average total protein yield from NK cells isolated from 5 human donor whole blood using Protocol 2. The Pierce BCA and Bio-Rad DC protein assays were simultaneously used to detect protein concentrations in each sample. Abbreviations: Bio-Rad DC protein assay (Lowry), detergent-based protein extraction method (Protocol 2), myelogenous leukemia cell line (K562 Cells), Pierce Bicinchoninic Acid protein assay (BCA), ultrasonic-based protein extraction method (Protocol 1).

Article Snippet: Furthermore, assessment of the two protein quantification assays was undertaken: The Pierce BCA and the Bio-Rad DC protein (Lowry) assay kits, to determine their sensitivity and compatibility with the two adapted protocols.

Techniques: Protein Extraction, Comparison, Membrane, Cell Culture, Spectrophotometry, Isolation, DC Protein Assay, Bicinchoninic Acid Protein Assay

Journal: Frontiers in Physiology

Article Title: Identification of transient receptor potential melastatin 3 proteotypic peptides employing an efficient membrane protein extraction method for natural killer cells

doi: 10.3389/fphys.2022.947723

Figure Lengend Snippet: Standard curve plot examples of the Bio-Rad DC (Lowry) protein assay and the Pierce BCA protein assay illustrating variations between the two protein assay standard curve plots using Protocol 2 buffers. (A) Compatibility illustration of the Bio-Rad DC protein assay kit with Protocol 2 lysis buffer: pre-precipitation, (B) protein solubilization detergent: post-precipitation. Different concentrations of BGG protein as reference protein standard diluted in lysis buffer and solubilization detergent respectively, were plotted against absorbance values. (C) Compatibility illustration of the Pierce BCA protein assay kit with Protocol 2 lysis buffer: pre-precipitation, (D) protein solubilization detergent: post-precipitation. Different concentrations of BSA protein as reference protein standard diluted in lysis buffer and solubilization detergent respectively, were plotted against absorbance values. Abbreviations: Bovine serum albumin (BSA), Bovine gamma globulin (BGG).

Article Snippet: Furthermore, assessment of the two protein quantification assays was undertaken: The Pierce BCA and the Bio-Rad DC protein (Lowry) assay kits, to determine their sensitivity and compatibility with the two adapted protocols.

Techniques: Bicinchoninic Acid Protein Assay, DC Protein Assay, Lysis